Once you have anĪccount, you can manage your list preference from this page. Subscribe to the list, click on "Subscribe or Unsubscribe" on this page. There is a mailing list for CCP-EM (supported by JISCMail). Support for software developers including porting, testing, and distribution of software.ĬCP-EM has been supported by the MRC since 2012 through a series of Partnership Grants.Support the users of software for cryo-EM through dissemination of information on available software, and directed training.Provide a focus for the cryo-EM community to interact withĬCP4/ CCPBioSim/ CCPN, and the broader international community. Build a UK community for computational aspects of cryo-EM.
Data represent mean value ± standard deviation.The Collaborative Computational Project for electron cryo-microscopy (CCP-EM) supports users and developers in biological EM. (CII) Succinate quinone dichlorophenol indophenol reductase (CII + CIII) succinate cytochrome c reductase (CIII) decylubiquinol cytochrome c reductase (CIV) cytochrome c oxidase (CS) citrate synthase (IDH) isocitrate dehydrogenase. ( D) Relative activities of respiratory chain and TCA cycle enzymes in control ( n = 5, white bars) and Phb2 −/− MEFs ( n = 4, black bars) after Cre-mediated inactivation of Phb2. Data represent mean value ± standard deviations. ( C) Oxygen consumption in permeabilized control ( n = 5 white bars) and Phb2 −/− MEFs ( n = 3 black bars) after Cre-mediated inactivation of Phb2 under conditions of substrate-driven respiration. Dissipation of the membrane potential with CCCP and unstainedĬells were used as controls. The fluorescent dye JC-1 and analyzed by flow cytometry at 590 nm. The cell lines indicated were stained with ( B) Maintenance of mitochondrial membrane potential in prohibitin-deficient MEFs. PHB2-specific, and, for control, β-actin-specific antibodies. Cell lysates were analyzed by SDS-PAGE and immunoblotting using OPA1-specific, PHB1-specific, ( A) Immunoblot analysis of Phb2 fl/fl and Phb2 fl/fl∷Phb2 cells transduced with Cre-recombinase. PHB2 controls cleavage of OPA1 but does not affect respiratory activities.
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